Luminex MasterPlex CT Tutorial Manual Manual do Utilizador

Tutorial for MasterPlex® CT v1.0 © 2006 Hitachi Software Engineering America, Ltd. All Rights Reserved.

http://www.miraibio.comPlex CT 10 Here is the breakdown and a brief summary of the wizards:  The Startup Wizard walks you through the warm up and

http://www.miraibio.comPlex CT 11 Please note that there are detailed instructions provided at each step. The next window checks to make sure that

http://www.miraibio.comPlex CT 12 In the next step of the Startup Wizard, you can perform the Prime procedure. Priming removes air bubbl

http://www.miraibio.comPlex CT 13 The next step takes you through the Alcohol Flush, which removes air bubbles from the cuvette. In this step, you

http://www.miraibio.comPlex CT 14 You are now done with the Startup Wizard and your Luminex system is ready for use. After finishing one wizard,

http://www.miraibio.comPlex CT 15 Action List Manager The Action List Manager lets you customize and automate routine procedures such as Startup, Ma

http://www.miraibio.comPlex CT 16 Next, we want to specify the number of times a step is to be repeated and the well location for the ope

http://www.miraibio.comPlex CT 17 Lastly, we will name our newly created Action List to “Startup Procedure” in the Action Name form and press the S

http://www.miraibio.comPlex CT 18

http://www.miraibio.comPlex CT 19 Three Step Acquisition MasterPlex CT v1.0 allows just three simple steps to run an acquisition: 1) What – Spe

http://www.miraibio.comPlex CT 2

http://www.miraibio.comPlex CT 20 Let’s begin with the Region settings. First click the Region Modify button to activate appropriate regions. Th

http://www.miraibio.comPlex CT 21 To define bead region names, click on the Properties tab. Next, double-click the default name und

http://www.miraibio.comPlex CT 22 Now, let’s enter our parameters that will determine how the machine will run by clicking the Setup Modify button.

http://www.miraibio.comPlex CT 23 In the Setup tab, you can specify the Double Discriminator gate values, the direction of which the acquisition

http://www.miraibio.comPlex CT 24 To mark your sample wells, you can simply drag and click to highlight wells or hold down the CTRL key to select

http://www.miraibio.comPlex CT 25 Congratulations! You have now completed the 3 steps needed to begin data acquisition. You have entered your: 1)

http://www.miraibio.comPlex CT 26 Master

http://www.miraibio.comPlex CT 27 Kit Manager MasterPlex CT has built-in capabilities to recognize predefined kit configurations so that you will n

http://www.miraibio.comPlex CT 28 You can use this Kit Manager to:  Import new kit files  View general information about a kit  Duplicate a

http://www.miraibio.comPlex CT 29 The Plate Map tab displays background, standard, and control sample locations. The Regions tab

http://www.miraibio.comPlex CT 3 In this tutorial, we will be highlighting 8 powerful features of the MasterPlex CT v1.0 software: 1) Probe Hei

http://www.miraibio.comPlex CT 30 The Samples tab displays the information regarding the standards, negative controls and quality control sampl

http://www.miraibio.comPlex CT 31 Click on the Cancel button to close the Lot Information window and then press the Apply button to use the kit co

http://www.miraibio.comPlex CT 32

http://www.miraibio.comPlex CT 33 Seamless Integration MasterPlex CT seamlessly integrates with other MasterPlex analysis packages such as MasterP

http://www.miraibio.comPlex CT 34 The analysis program you have chosen automatically launches. If you have used a kit configuration file, all

http://www.miraibio.comPlex CT 35 Select the data type pull menu and view the Concentration data. All the unknown concentrations for each

http://www.miraibio.comPlex CT 36 To view the Standard Curve graph, click on the Standard Curve button. To view the x-axis in log scale, rig

http://www.miraibio.comPlex CT 37 To view multiple standard curves in the same graph, hold down the CTRL key and select the reg

http://www.miraibio.comPlex CT 38 MasterPlex CT has many different options to view data. For example, to view a graph in 3-D, click on the 3D b

http://www.miraibio.comPlex CT 39 Fail Safe Acquisition With MasterPlex CT, your data acquisition need not be “all or nothing.” The Fail Safe Acqu

http://www.miraibio.comPlex CT 4

http://www.miraibio.comPlex CT 40 While the data is being reprocessed, the data is presented in the same view as if it was being acquired in real

http://www.miraibio.comPlex CT 41 If you discover that you are not using optimal settings during the run, cl

http://www.miraibio.comPlex CT 42

http://www.miraibio.comPlex CT 43 Remote Monitor Free yourself from the instrument. MasterPlex CT includes a remote client program that allows yo

http://www.miraibio.comPlex CT 44 In case of an emergency, the Luminex instrument can be remotely paused by just simply clicking on the Pause but

http://www.miraibio.comPlex CT 45 In the Data tab, you can view MFI data as it is being acquired well-by-well in real-time. In the Co

http://www.miraibio.comPlex CT 46 In the Device tab, you can monitor the status of the Luminex instrument. In the abo

http://www.miraibio.comPlex CT 47 You can also contact MiraiBio with any questions you have at (510) 337-2000 (USA phone number) or by sending an e

http://www.miraibio.comPlex CT 5 Probe Height Adjustment Being able to adjust the probe height is crucial for any Luminex user as it is the cause

http://www.miraibio.comPlex CT 6 Thumbwheel Bolt The initial Adjust Probe window gives detailed instructions on adjusting the probe he

http://www.miraibio.comPlex CT 7 4. After you have placed the appropriate alignment tools into the well, press the Location button and specify the

http://www.miraibio.comPlex CT 8

http://www.miraibio.comPlex CT 9 Wizard Interface MasterPlex CT provides a set of wizard driven interfaces to help familiarize new users with the s