Luminex MasterPlex QT Tutorial Manual Manual do Utilizador descarregar pdf (Página 44)

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b. Fold Change – If the With normalization check box is ticked, it will perform

a normalization step before the fold change calculation between the treatment and

control groups.

You have 3 options when normalizing:

1. Normalize by a single house keeping gene.

2. Normalize by the geometric mean of all the housekeeping genes

selected in Step 1 if 2 or more genes were selected.

3. Normalized by a Constant value.

c. Normalization – This performs the data normalization step only using one of

the 3 options discussed above.

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Luminex MasterPlex QT Tutorial Manual Manual do Utilizador Página 44