Luminex FLEXMAP 3D Hardware User Manual Manual do Utilizador

FLEXMAP 3D® HardwareUser Manual

Table of ContentsChapter 1 Safety ...1I

Chapter 3 Maintenance and Cleaning ...23General Maintenance Precautions ...

Become familiar with the information in this chapter before using the equipment. Do notperform procedures on your system that are not specifically con

SymbolsYou may encounter these symbols during the use and operation of the Luminex® FLEXMAP3D® system. They represent warnings, conditions, identifica

Testing and CertificationThe FLEXMAP 3D system has been tested by MET.FIGURE 1.MET MarkIn addition, Luminex FLEXMAP 3D complies with European Union (E

GeneralCAUTION: Keep access doors closed during normal operation.Always observe standard laboratory safety practices.Electromagnetic CompatibilityThe

FIGURE 4.Avoid Exposure LabelAll Class 3b laser apertures are located within the FLEXMAP 3D instrument and arecontained within a protective housing, a

salts” (Centers for Disease Control, Atlanta, Georgia, April 30,1976).Biological SamplesHuman and animal samples may contain biohazardous infectious a

Electrical ComponentsDo not perform any maintenance or cleaning of the electrical components in the system,with the exception of replacing fuses.The f

HeatThe heater plate, used to warm the heater block of the XY platform, can be heated between35°C and 60°C.WARNING: The heater plate of the FLEXMAP 3D

Copyright Information© Luminex Corporation, 2011. All rights reserved. No part of this publication may bereproduced, transmitted, transcribed, or tran

FIGURE 7.Installed FLEXMAP 3D SystemDisposal of InstrumentWithin the European Union, the Waste Electrical and Electronic Equipment Directive2002/96/EC

FLEXMAP 3D® Hardware User Manual10

This chapter reviews the technical aspects of theLuminex® FLEXMAP 3D® system: operation,system components, subsystems, recommended additional equipmen

ElectronicsThe electronics system provides the power for operation and control of the FLEXMAP 3Dsystem and communication between its parts.Power Input

1 Power Input Module2 Communications Port P1 (USB type B)FluidicsThe fluidics system handles the flow of liquid through the Luminex® FLEXMAP 3D®instru

Cheminert® FittingThis fitting attaches the sample probe to the sample tubing. Disconnect this fitting when youremove the sample probe. See the "

FIGURE 11.Syringe Pump1 Syringe arm (within dotted lines)2 Glass syringe barrel3 Syringe plungerSheath FilterThe sheath filter removes particles great

FIGURE 12.Fluidics Bay1 Sheath filter2 Tubing to sheath fluid container is attached here (blue)3 Tubing to waste fluid container is attached here (ora

of this manual. The "Fluidics Bay" image above and "Front Door Ventilation Filters" imagebelow show the location of these filters.

2 Holding clamp3 Cover over HEPA air filterOpticsThe optical system consists of the optical assembly and the excitation lasers. The opticalcomponents

Standard Terms and Conditions for Use of InstrumentProductBy opening the packaging containing this product ("Product") or by using such Prod

CAUTION: Adhere to standard laboratory safety practices when handlinghazardous, toxic, or flammable reagents and chemicals. ContactLuminex Technical S

Uninterruptible Power Supply (UPS) or Surge ProtectorLuminex recommends using either an uninterruptible power supply (UPS) or a surgeprotector to prot

Pollution degree 2Operating temperature: 15°C to 30°C (59°F to 86°F)Operating humidity: 20% to 80%, noncondensingShipping and storage temperature: 0 t

Classification detector: Avalanche photo diodes with temperature compensationDoublet discrimination detector: Avalanche photo diodes with temperature

To ensure accurate test results, properly clean and maintain the Luminex® FLEXMAP 3D®system. Read and follow all instructions in this section. Perform

Most of the daily maintenance tasks for the FLEXMAP 3D system, including systeminitialization, warmup, and shutdown, can be performed using available

overflow. Empty the waste fluid container each time you replace or fill the sheath fluidcontainer.Place the waste fluid container at least three feet

Removing ClogsIf you frequently use the FLEXMAP 3D system to test concentrated serum or otherdebris ridden samples, Luminex recommends that you use th

4. Replace the sample probe and tightly screw in the Cheminert fitting.5. Use the software to perform an automatic probe height adjustment. NOTE: Perf

1. Turn off the FLEXMAP 3D instrument and unplug the power cord.2. Wipe all exterior surfaces with mild detergent, followed by a household bleach solu

hardware not provided by Luminex. If Product is purchased from a Luminex authorizedreseller, any warranty obligations shall be provided in writing dir

13. Use the software to run the prime command twice, watching for any leaks in the syringearea.14. Close the right front door. FIGURE 16.Syringe Assem

5. Remove the filter with one hand and hold the tubing with the other hand. CAUTION: Do not allow the tubing to fall inside the instrument.6. Connect

6. Stand the filters upright to air dry. CAUTION: Filters must be completely dry prior to reinstallation.7. Reinstall filters. The arrows on the door

Annual maintenance requires replacing the sheath filter.To replace the Luminex® FLEXMAP 3D® instrument sheath filter:1. Turn off the FLEXMAP 3D instru

Storing the FLEXMAP 3D InstrumentStoring the FLEXMAP 3D InstrumentThis procedure explains the steps you should take before placing the FLEXMAP 3Dinstr

DANGER: To avoid serious injury or death by electric shock, you must turnoff the FLEXMAP 3D instrument and unplug it from the wallbefore replacing a f

Maintenance LogsReproduce the following forms as necessary and use them to record maintenanceinformation. Fill in the dates in the first line of the t

Semi-annualMaintenanceClean ventilationfiltersReplace HEPA airfilterReplace syringesealsAnnualMaintenanceReplace sheathfilterFLEXMAP 3D® Hardware User

Troubleshooting procedures help users identify and remedy problems with the instrument.OverviewTo troubleshoot a problem, locate the symptom in one of

Power Supply ProblemsPower supply problems often involve a blown fuse, faulty electronic component, ordisconnected cable.CAUTION: Whenever you deal wi

indicated on the Product label, the Product has not received approval from the United StatesFood and Drug Administration or other federal, state or lo

ClogsOften, a clog somewhere in the Luminex® FLEXMAP 3D® instrument is the cause of a fluidleak, a pressurization or sample probe problem, or a proble

Fluid LeaksFluid leaks can result in poor pressurization and failed sample acquisition.TABLE 4.Fluid LeaksSymptom Possible cause SolutionFluid is pool

Symptom Possible cause SolutionThe microtiter plate is warped. Inspect the microtiter plate.Replace it if it is warped.The sample probe is bent. Remov

Symptom Possible cause SolutionThere is air in the instrument. Thewaste line was moved duringinstrument operation, resulting inan unstable flow rate.P

Symptom Possible cause SolutionThe verification lot is expired. Use an unexpired bottle ofverification microspheres.The verification microsphereshave

Symptom Possible cause SolutionThe wrong lot number or targetvalues are entered in theappropriate page of the softwareUI.Check the lot number and targ

FIGURE 22.Normal Bead DetailTABLE 9.Bead Detail IrregularitiesSymptom Description Possible Problem SolutionxMAP and calibrator microspheres form a gro

Symptom Description Possible Problem SolutionxMAPmicrospheres donot form acohesive groupand aresomewhatscattered.There is air in theinstrument.Verify

ShippingIf a serious problem arises with the FLEXMAP 3D system, it may be necessary to return it toLuminex Corporation for repairs. If Luminex Technic

6. Connect the waste fluid tubing (orange connector) to the bottom quick disconnect point(white connection) of the sheath filter tubing.7. Use the sof

contract or any other theory of law or equity arising out of, directly or indirectly, the use of theProduct or by reason of Buyer's failure to pe

YesNoPrinted Name _____________________________________________________Signature ________________________________________________________Company/Insti

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Installation of the FLEXMAP 3DThe following installation instructions for FLEXMAP 3D are to be used for site prep,assessing facilities requirement and

1. Place the PC and monitor on the right side of the instrument.2. Connect the FLEXMAP 3D instrument and all necessary peripheral devices to the PC. F

1 Power Inlet 2 PC Communications plug (USB type B)3. Connect the USB communications cable between the FLEXMAP 3D instrument (P1) andthe PC, and conne

18. Calibrate the probe height using the instructions in the software user manual. NOTE: For information on calibrating the probe correctly, refer to

Installation55

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HardwareProduct Description Customer NumberLuminex® FLEXMAP 3D® with xPONENT 4.0 FLEXMAP-3DHeater Block for 96-well microtiter plates CN-0224-01Heater

SoftwareProduct Description Customer NumberLuminex xPONENT 4.0 Software for FLEXMAP 3D CN-SW16-01ReagentsProduct Description Customer NumberxMAP Sheat

End-User License Agreement (EULA) for Luminex®xPONENT® SoftwareThis Luminex End-User License Agreement (“EULA”) is a legal agreement between you(eithe

NOTE: The Probe Height Adjustment Tool is to be used for 384 well mylarbottom and 384 well filter bottom plates only.To calibrate the probe correctly,

1. Using data sheet for the plate you intend to calibrate the probe to, subtract the "welldepth" from the "plate height" as shown

2.9mm or well D7 on the probe height adjustment tool for this example.FLEXMAP 3D Probe Height Adjustment Tool61

3. GRANT OF LICENSE. Subject to the terms and conditions of this EULA, Luminex herebygrants to you a nonexclusive, nontransferable, nonassignable lice

5. TERM AND TERMINATION. Your rights under this EULA are effective until termination.You may terminate this EULA at any time by destroying the SOFTWAR